*Electrophysiology of Plant and Bacterial MS Channels

We have used single channel patch-clamp electrophysiology to characterize E. coli MscS and Arabidopsis MSL8 and MSL10 expressed in Xenopus laevis oocytes. These studies establish that MSLs are bonafide mechanosensitive ion channels, and their behavior in oocytes closely resembles MSL-dependent activities in Arabidopsis root and pollen protoplasts. Differences in the behavior of MSL10, MSL8 and MscS may have implications for their respective roles in plant and bacterial physiology.