December 11, 2014

Introduction to Microelectrode Arrays, the Site-​Selective Functionalization of Electrode Surfaces, and the Real-​Time Detection of Binding Events

Graaf, Matthew D. and Moeller, Kevin D.

Langmuir, 2014, Ahead of Print

A review.  Microelectrode arrays have great potential as anal. tools because currents can be independently measured at each electrode in the array.  In principle, these currents can be monitored to follow in real time the binding events that occur between the members of a mol. library and a biol. target.  To capitalize on this potential, the surface of the array must be selectively functionalized so that each unique member of the mol. library is assocd. with a unique individually addressable electrode or set of electrodes in the array.  To this end, this instructional review summarizes methods for coating the arrays with porous polymers that allow for the attachment of mols. to the surface of the array, selectively conducting reactions at individual electrodes in the array, characterizing mols. that are placed on the arrays, and running the anal. expts. needed to monitor in real time binding events between mols. on the array and a biol. target.