August 24, 2016

New Methods for the Site-Selective Placement of Peptides on a Microelectrode Array: Probing VEGF-v107 Binding as Proof of Concept

Cu(I)-catalyzed "click"-reactions cannot be performed on a borate ester derived polymer coating on a microelectrode array because the Cu(II)-precursor for the catalyst triggers background reactions between both acetylene and azide groups with the polymer surface.  Fortunately, the Cu(II)-background reaction can itself be used to site-selectively add the acetylene and azide nucleophiles to the surface of the array.  In this way, mols. previously functionalized for use in "click"-reactions can be added directly to the array.  In a similar fashion, activated esters can be added site-selectively to a borate ester coated array.  The new chem. can be used to explore new biol. interactions on the arrays.  Specifically, the binding of a v107 derived peptide with both human and murine VEGF was probed using a functionalized microelectrode array.

By: Graaf, Matthew D.; Marquez, Bernadette V.; Yeh, Nai-Hua; Lapi, Suzanne E.; Moeller, Kevin D.

ACS Chem. Biol., 2016, 11 (10), pp 2829–2837
Publication Date (Web): August 24, 2016